Fig. 11.

Effect of CM764 treatment on expression of VEGF in SK-N-SH neuroblastoma. Cell lysates of SK-N-SH neuroblastoma cells were made after treatment with 10 μM CM764 for the indicated amount of time and were used for western blotting for VEGF as described in Materials and Methods. (A) VEGF western blot and β-tubulin loading control. (B) Independent blots were quantified and the ratio of the VEGF/loading control (β-tubulin or glyceraldehyde-3-phosphate dehydrogenase) was determined and averaged for all experiments. Data are expressed as fold increase in ratio relative to control treated without CM764. Treatment with CM764 induced protein expression of VEGF after 24-hour exposure, following an earlier increase in HIF1α expression as shown in Fig. 10 (one-way analysis of variance F = 6.558, Dunnett’s test for multiple comparisons compared with untreated control **P < 0.01). Results shown are an average of three independent experiments. A representative blot is shown.