Fig. 6.

Effect of CM764 treatment on intracellular calcium in SK-N-SH neuroblastoma. Cells were loaded with fura-2 acetoxymethyl ester for 60 minutes prior to CM764 or vehicle injection (injection indicated by the black arrow). CM764 injection induced an immediate increase in cytosolic calcium in SK-N-SH neuroblastoma, as has been previously demonstrated in response to sigma-2 receptor activation. A representative trace for each condition is shown. Data are presented as a change in intracellular calcium level with the baseline removed, with the baseline level of calcium determined by the average of 25 ratio measurements prior to injection. The experiment was repeated four times with similar results, with each experiment having four replicates per condition.