Table 2.
Comparison of the sensitivity of virus isolation, the 3 molecular diagnostic tests and the 5 RAD tests in the detection of EI virus in three experimentally infected horses
| Post‐infection day | Clinical signs* | Tests | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Virus isolation** | Conventional RT‐PCR | rRT‐PCR*** | RT‐LAMP | ImmunoAce Flu | BD Flu examan | Quick chaser Flu A, B | ESPLINE Influenza A&B‐N | Prorast Flu | ||
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 1 | 0 | 3 | 3 | 3 | 3 | 1 | 0 | 1 | 0 | 0 |
| 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| 3 | 3 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 2 |
| 4 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 0 |
| 5 | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 3 | 2 | 0 |
| 6 | 3 | 2 | 3 | 3 | 3 | 2 | 2 | 2 | 1 | 1 |
| 7 | 3 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 |
| 8 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| 9 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
The days of sample collection are listed in the first column. The remaining columns list the number of horses that tested positive by each assay.
*If fever (≥38·5°C) and/or nasal discharge and/or coughing was observed, the horse was scored as positive for clinical signs.
**The lowest virus titre of the virus isolation was 1·4 log EID50/ml.
***The lowest RNA copy number of rRT‐PCR was 5 × 103 copies/ml (10 copies/test).