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. 2016 Jan 29;10(2):134–140. doi: 10.1111/irv.12365

Figure 1.

Figure 1

SDS–PAGE analysis of purified br‐HA from H7N9 A/Shanghai/2/2013 and H7N3 A/mallard/Netherlands/12/2000 vaccine viruses. Virus and fractions from the sucrose gradient following bromelain treatment were denatured and subjected to SDS–PAGE under reducing conditions. (A) Virus and br‐HA fractions prepared from H7N9 A/Shanghai/2/2013 vaccine virus. (B) Virus and br‐HA fractions prepared from A/mallard/Netherlands/12/2000 vaccine virus. Lane 1 – purified virus before bromelain treatment; lane 2 – fraction 6 from the 5–20% continuous sucrose gradient; lane 3 – fraction 7. (C) Infected cell extract (lane 1) or supernatant (lane 2) from cells infected with modified vaccinia virus Ankara (MVA) vector expressing H7N9 A/Shanghai/2/2013 HA subjected to SDS–PAGE followed by Western blotting with an H7 monoclonal antibody. Molecular weight markers (M) are shown in kDa.