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. 2016 Feb 9;6:19791. doi: 10.1038/srep19791

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Copyright © 2016, Macmillan Publishers Limited

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(A) HPLC time series of D-glucose, D-glucose and n-butyl alcohol, phthalic acid and n-butyl alcohol, protocatechuic acid and n-butyl alcohol for cases when the substrates were catalyzed by crude enzyme from the three different species of filamentous fungi: (a) T.asperellum PTN7; (b) A.niger PTN42; and (c) P.lanosum PTN121. Black arrows indicate the corresponding HPLC peaks of DBP (See controls in Supplementary Fig. S3, controls 2–7). (B) Biosynthesis of DBP catalyzed by crude enzyme from the three different species of filamentous fungi with different substrates: (a) phthalic acid and n-butyl alcohol; (b) protocatechuic acid and n-butyl alcohol; (c) D-glucose and n-butyl alcohol; and (d) D-glucose. (C) DBP biosynthesis of the filamentous fungi, T.asperellum PTN7, A.niger PTN42, and P.lanosum PTN121.