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. 2016 Feb 9;6:20733. doi: 10.1038/srep20733

Figure 5. ON/OFF control of gene expression using ASODN/RNase H-MNP conjugate (Type II).

Figure 5

Cell-free protein synthesis mixtures were incubated at 30 °C in the absence (grey circles) or presence (blank circles) of the ASODN/RNase H-MNP conjugate. In a parallel experiment, ASODN/RNase H-MNP conjugate was added to the reaction mixture at 45 min and removed at 90 min (black circles). Fluorescence of cell-free synthesized sfGFP was measured every 20 min. Error bars in the graph indicate standard deviations from three independent reaction samples.