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. 2016 Feb 9;6:20453. doi: 10.1038/srep20453

Figure 1. Loss of Bif-1 induces obesity.

Figure 1

(a) Female wild type (WT) and Bif-1 knockout (KO) mice were fed with either control chow diet (CD) or high-fat diet (HFD) for the indicated periods of time. Body weight of each mouse was monitored weekly (n = 9–12). Statistical significance was determined using one-way ANOVA followed by Bonferroni’s multiple comparison test. (b) Representative images of WT and Bif-1 KO mice fed on HFD for 23 weeks. (c–e) Body composition of mice fed with the indicated diet for 23 weeks was measured using TD-NMR. Loss of Bif-1 leads to larger total (c) and percent (d) fat mass and has no effect on lean mass (e). Statistical significance was determined using one-way ANOVA followed by Bonferroni’s multiple comparison. (f) Representative images of fat deposits in WT and Bif-1 KO mice fed on HFD for 23 weeks. (g) Representative images of abdominal gonadal fat pads from WT and Bif-1 KO mice fed on either CD or HFD for 23 weeks. (h,i) Loss of Bif-1 leads to adipocyte enlargement. Hematoxylin and eosin stain of the abdominal gonadal white adipose tissue (WAT) (h) and interscapular brown adipose tissue (i) sections from mice fed with CD or HFD for 23 weeks. Scale bars in (h) and (i) represent 50μm and 200μm, respectively. All values are mean ± SEM. Differences with controls were significant for *p < 0.05, **p < 0.01, and ***p < 0.001.