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. 2016 Feb 9;6:20453. doi: 10.1038/srep20453

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Copyright © 2016, Macmillan Publishers Limited

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Indirect calorimetry analysis of WT and Bif-1 KO mice fed with CD for 4 weeks (10-week old, n = 4) (a,b,e) or with the indicated diet for 19 weeks (25-week old, n = 8) (c,d,f). Food intake (a,c), physical activities (b,d), and energy expenditure (e,f) were measured during a 48-h cycle using metabolic cages. Statistical significance was determined using one-way ANOVA followed by Bonferroni’s multiple comparison. All values are mean ± SEM. Differences with controls were significant for *p < 0.05 and ****p < 0.0001.