Figure 6. Bif-1 is important for autophagy in adipose tissue.

(a–c) Immunoblots for the indicated proteins in WAT homogenates prepared from 30-week-old mice. The mice were fed with CD or HFD for 23 weeks (a), fed with CD, at basal or 16 h-fasted states (b), or fasted for 16 h, with or without insulin injection 15 min prior to sacrifice (c). n = 3–4. (d) Immunoblots for the indicated proteins in WAT homogenates from 6-week old mice fed with CD or 11-week old mice fed with 5 weeks HFD. n = 3.