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. 2016 Jan 26;26(2):171–189. doi: 10.1038/cr.2016.15

Figure 6.

Figure 6

WLS and SFRP2 are major transcriptional targets of SOX2, and mediate SOX2's function in the control of Wnt signaling in hESCs. (A) Integrative Genomics Viewer screenshots showing common SOX2 peaks on the promoters of SFRP2 and WLS in hESCs and hNPCs. Two WLS peaks are named WLS-1 and WLS-2. (B) RT-qPCR results of SFRP2 and WLS along with other Wnt pathway components at day 1, 2 and 3 after SOX2/3 KD in hESCs. (C) Western blot results of SFRP2 and WLS at day 1 and day 2 after SOX2/3 KD in hESCs with or without IWR1-e (10 μM). (D) Morphology of H9 hESCs infected with lentiviral shRNA (shNT or shWLS) in combination with NT οr SOX2/3 KD using siRNA (siNT or si2/3-1). Recombinant SFRP2 was supplemented at 200 ng/ml. (E) RT-qPCR results of germ layer markers and Wnt signaling components in cells from D. Statistical analysis is performed as follows: the 3 rescue approaches within the si2/3-1 group are compared with the rescue effect of the vehicle (shNT+vehicle); the shNT+vehicle in si2/3-1 group is compared with the shNT+vehivle in the siNT group, whose values are set as 1.