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. 2016 Feb 9;6:20845. doi: 10.1038/srep20845

Figure 5. LPS induced NFκB activation in PLTP deficient macrophages and PLTP knockdown RAW264.7.

Figure 5

Bone marrow derived macrophages (BMDM) or RAW264.7 were stimulated with LPS (200 ng/mL) for 0, 30, 60, 120 min. The nuclear and cytoplasmic extracts were determined with anti-p65 and anti-IκBα, respectively. Anti-histone 3 (H3) and anti-GAPDH antibodies were employed as nuclear and cytoplasmic protein loading controls, respectively. PLTP (A) Nuclear p65 and cytoplasmic IκBα in BMDM from wild type mice (WT) VS. PLTP knockout (PLTP−/−). (B) Densitometry analysis of nuclear p65 and cytoplasmic IκBα in BMDM. (C,D) the mRNA level and protein level of PLTP knockdown via siRNA (150 pmol/L for PLTP siRNA and control siRNA, respectively) transfection. (E) Nuclear p65 and cytoplasmic IκBα from control siRNA or PLTP siRNA treated RAW264.7. (F) Densitometry analysis of nuclear p65 and cytoplasmic IκBα in RAW264.7. These results are a representative of 3 independent experiments. *P < 0.05; **P < 0.01; NS, no significance.