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. 2016 Feb 9;6:21499. doi: 10.1038/srep21499

Figure 3. Osteoclasts sealing zone formation and bone resorption activity at different stages.

Figure 3

(A) Representative images of FAK stain of osteoclasts at different stages during differentiation. Sealing zone was observed at lower layers with confocal microscopy. The scale bar represents 20 μm. (B) Representative images of pit formation assay. Cells were induced with RANKL (100 ng/mL) and M-CSF (50 ng/mL)for 0 h, 24 h, 72 h and 96 h.The scale bar represents 800 μm. (C) Number of fusing osteoclasts at different stages. (D) Average nuclei number of osteoclasts at different stages in FAK stain. (E) Quantification of pit area formation in (B). The data in the figures represent the averages ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001 based on one-way ANOVA.