Figure 4.

Reciprocal Mutations in the TIR(83,85,86) and NB(288) Regions of L7 and L6 Have Complementary Effects on Effector-Dependent Signaling Activity.

(A) Cell death scoring scale from 0 (no cell death, pale-yellow color) to 4 (confluent cell death, red color).

(B) Cell death activity of L7 mutants.

(C) Cell death activity of L6 mutants.

(B) and (C) Graphs representing effector-dependent cell death activity of L7 and L6 mutants, fused to YFP, represented as a percentage of infiltrated panels producing each cell death score (color-coded bars as indicated in [A]) 3 d after infiltration. Each mutant was tested in at least three independent infiltration experiments in transgenic tobacco W38 expressing AvrL567 together with L6 and L7 as controls on the same leaf. For each mutant, the total number of scored leaves is indicated in parentheses following each corresponding construct on the abscissa axis. Location of mutations is indicated above the scoring bars with black straight line defining mutations within the TIR, NB, ARC1, and ARC2 domains or ARC2/LRR spacer region and dashed arrows defining mutations in residues polymorphic between L6 and L7 or polymorphic between L6 and L2, L5, and L10.