Figure 3.

Transcriptional SAR Response in Distal Leaves of Plants Inoculated in Primary Leaves with Psm (OD₆₀₀ = 0.005) at 48 HAI.

Six independent SAR assays for Col-0 and three independent SAR experiments for both sid2 and ald1 were performed. Gene expression was analyzed by RNA-seq analyses of the resulting replicate samples for Psm and mock treatments at the whole-genome level.

(A) Venn diagram depicting numbers of differentially regulated genes between Psm and mock treatments of Col-0 (black), sid2 (red), and ald1 (blue) (FDR < 0.01). Overlap of genes is indicated. Left: significantly upregulated genes (the Col-0 genes correspond to the SAR⁺ genes). Right: significantly downregulated genes (the Col-0 genes correspond to the SAR⁻ genes). Note that only two genes are differentially regulated in ald1.

(B) Distribution of P/M-fold changes of SAR genes in Col-0, sid2, and ald1. Box plots depict log₂-transformed P/M-fold changes. The distribution of log₂ P/M-fold changes for three sets of randomly selected genes (left, 3413 genes; right, 2893 genes) is included (random a, b, and c). Left, SAR⁺ genes; right, SAR⁻ genes.