Figure 1.

Overexpression of BAG6 in Arabidopsis Induces Lesion Mimic Development.

Arabidopsis bag6-1 T-DNA insertion mutant was transformed with N-terminal HA- or YFP-tagged BAG6 under either 35S promoter or native promoter (NP) by the floral dip method. Stable transgenic plants harboring BAG6 driven by the 35S promoter (35S:HA-BAG6 and 35S:YFP-BAG6) exhibited apparent lesion mimics on leaves ([D] and [F]), while plants expressing BAG6 driven by the native promoter (NP:HA-BAG6 and NP:YFP-BAG6) ([C] and [H]) were similar to wild-type Col-0 and bag6-1 mutant ([A] and [B]). Overexpression of a cleavage-deficient form of BAG6, BAG6^D760A, driven by 35S promoter (35S:HA-BAG6^D760A and 35S:YFP-BAG6 ^D760A), abolished the lesion mimic phenotype in the bag6 mutant background ([E] and [G]). Representative plants were photographed. White circles show the typical lesion mimic phenotype.