Figure 6.

BAGP1 and APCB1 Are Required for Disease Resistance and BAG6 Cleavage.

(A) Detached leaves of wild-type Col-0, bag6-1, bagp1-1, and apcb1-1 mutants were agar plug inoculated with B. cinerea. Lesion development was monitored and representative photographs were taken 48 h after inoculation.

(B) Lesion diameters were measured at 24 and 48 h after inoculation. Data represent means ± sd from three replicates.

(C) Arabidopsis Col-0, bagp1, and apcb1 protoplasts were transfected with either HA-AtBAG6 or HA-AtBAG6^D760A under 35S promoter in the presence or absence of chitin (lanes 1 to 4 and 6). BAG6 cleavage was monitored using immunoblotting. BAG6 cleavage was restored when the bagp1 and apcb1 mutants were complemented (lanes 5 and 7). A catalytically inactive form of APCB1 (Myc-APCB1^D223A/D431A) was unable to restore BAG6 cleavage (lane 8). Equal protein samples were separated by 10% SDS-PAGE and stained with Coomassie blue (CBS); the presence of HA-BAG6 was detected by an anti-HA antibody.