Figure 7.

BAG6 Induces Autophagic Structures in Plants.

(A) Wild-type Col-0, bag6 mutant, and the autophagy-deficient mutant atg18 (SALK_081770C) were inoculated with B. cinerea using colonized agar plugs, and lesions monitored over time. Photographs of representative leaves were taken 48 h after inoculation.

(B) Lesion diameters were measured 24 and 48 h after inoculation with B. cinerea. Data represent means ± sd from three replicates.

(C) Autophagic activity was detected using the fluorescent stain MDC. Leaves were vacuum-infiltrated with a 100 μM final concentration of MDC for 30 min at 37°C, 24 h after B. cinerea inoculation. Fluorescence was visualized using an Olympus IX81 inverted fluorescence confocal microscope with excitation and emission wavelengths of 335 and 508 nm, respectively. All images were collected using Olympus DP controller and processed using Olympus Fluoview software. Bar = 20 μm.

(D) Representative TEM images of tobacco tissues expressing the full-length and cleaved form of AtBAG6. Tissues were processed 48 h after infiltration. Arrows indicate autophagosomal structures. Bar = 2 μm.