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. 2015 Dec 24;28(1):28–41. doi: 10.1105/tpc.15.00854

Figure 4.

Figure 4.

PKL, SWN, and CLF Promote the Deacetylation and/or Trimethylation of H3K27.

(A) Genomic location of the sites analyzed by ChIP.

(B) and (C) ChIP analysis of H3K27me3 (B) and H3K27ac (C) in the shoot apices of Col and mutant plants at 1, 2, and 3 weeks. pkl and swn-3 pkl have reduced levels of H3K27me3 at 2 and 3 weeks and elevated levels of H3K27ac at 1 and 2 weeks. Data are presented as the ratio of (MIR156A/H3) to (STM/H3) or (MIR156C/H3) to (STM/H3). Values are mean ± se for the technical replicates from one representative experiment.

(D) ChIP analysis of H3K27me3 in the shoot apices of 2-week-old Col and mutant plants. swn-3 had no detectable effect on H3K27me3, while clf-28 reduced H3K27me3 by ∼40% at MIR156A and by ∼25% at MIR156C. Values are mean ± se from three biological replicates for swn-3 and from one experiment for clf-28; asterisk indicates significant difference from Col, P < 0.01, Student’s t test.