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. 2015 Dec 24;28(1):28–41. doi: 10.1105/tpc.15.00854

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Figure 5. — PKL and PRC2 Bind to MIR156A and MIR156C.

(A) Location of the genomic sites analyzed by ChIP.

(B) Sites associated with PKL-FLAG in MIR156A and MIR156C chromatin in shoot apices of 1-, 2-, and 3-week-old PKL-FLAG plants, relative to non-transgenic plants. PKL binds in the promoter, near the TSS of MIR156A and MIR156C.

(C) Sites associated with FIE-HA in MIR156A and MIR156C chromatin in shoot apices of 1-, 2-, and 3-week-old FIE-HA plants, relative to nontransgenic plants. FIE binds near the TSS of MIR156A at 2 weeks and binds in a broader region of MIR156C at 2 and 3 weeks. Fold enrichment of each fragment was first calculated as the ratio of PKL-Flag to Col or FIE-HA to C24 and then normalized against a nonbinding site within MIR156A/MIR156C (A10 and C8 for PKL-Flag; A2 and C1 for FIE-HA). Values are presented as mean ± se for the technical replicates from one representative experiment.