Figure 2.

RAP2.2 and RAP2.12 Act Redundantly as Direct Inducers of Hypoxia-Responsive Genes.

(A) RT-qPCR analyses of five HRGs in 7-d-old ERF-VII double or single knock out seedlings and respective wild types under hypoxia and normoxia.

(B) RT-qPCR analyses of the same HRGs in mesophyll protoplasts from A. thaliana Col-0 transiently expressing either p35S:HA-GFP or p35S:(MA)RAP2.2-HBD. Chx or the solvent DMSO was applied 30 min before Dex or the solvent ethanol, and protoplasts were incubated for an additional 4 h under LD conditions. Transcript levels were normalized to ELONGATION FACTOR 1A (EF1a) mRNA. Values are means ± sd from three biological replicates (each with three technical replicates). Different letters indicate values that vary significantly at P < 0.05 (Tukey HSD test).