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. 2015 Dec 14;28(1):160–180. doi: 10.1105/tpc.15.00866

Figure 5.

Figure 5.

A LBD41 Promoter 33-bp Region Is Responsive to Hypoxia and ERF-VII Activation.

(A) Stable A. thaliana lines bearing a triplicated 33-bp region of the LBD41 promoter fused to a 35S minimal promoter and a LUC coding sequence (3x33bpmin:LUC). Hypoxia-dependent bioluminescence upon d-luciferin application is seen only in leaves of 3x33bpmin-containing reporter lines after 9 h of hypoxia, whereas no signal is observed in leaves of Col-0.

(B) Comparison of LUC activity from the 589-bp-long LBD41prom5′-2:LUC with the 3x33bpmin:LUC reporter construct in the presence of transiently expressed HA-ERF-VIIs in mesophyll protoplasts from Col-0. Data are means ± sd of six replicates; asterisks indicate significant differences from basal promoter activity at ***P < 0.001 and *P < 0.05 (Tukey HSD test). Fold induction is the ratio between the transcription factor-induced and basal promoter activity using p35S:HA-GFP as the control. Promoter activity values (LUC activity normalized to p35S:RUC activity) are shown in Supplemental Figure 4.