Functional Similarity between the Arabidopsis HRPE and Arabidopsis ADH1
ARE.
(A)
Y1H assay with stable 3xARE:HIS and 3xATCTA:HIS yeast strains transiently expressing AD-ERF-VII fusions. Water and an AD-only expressing vector were used as negative controls. Panels show equally concentrated yeast transformants and 1:10- and 1:100-fold dilutions after 5 d of growth on selective medium in the absence and presence of 40 mM of the HIS gene inhibitor 3-AT.
(B) Effect of HA-RAP2.2 and HA-RAP2.12 on 3x33bpmin:LUC in comparison to 3xAREmin:LUC and 3xATCTAmin:LUC in transiently transformed mesophyll protoplasts from A. thaliana Col-0, cotransfected with p35S:RUC. Data are means ± sd of six replicates; asterisks indicate significant differences from wild-type-induced promoter activity at **P < 0.01 (Tukey HSD test). Normalized promoter activity is LUC activity normalized to p35S:RUC activity.