Figure 5.

The ER-Localized Fusion Protein CYP81F2-RFP Focally Accumulates at Plant-Microbe Interaction Sites.

(A) and (B) CLSM images from transgenic leaf epidermal cells expressing CYP81F2-RFP under control of the endogenous promoter show no detectable RFP fluorescence in unchallenged epidermal cells (A) but strong fluorescence particularly under the infection site in pathogen treated ones 20 hpi with Bgh (B).

(C) CLSM image of transgenic cells coexpressing CYP81F2-RFP and CFP-labeled ER lumen demonstrates colocalization of both proteins to the ER 18 hpi with Bgh.

(D) CLSM analysis of transgenic leaf epidermal cells coexpressing CYP81F2-RFP and PEN2-GFP-TA_PEN2 indicates close proximity of ER accumulation and PEN2 aggregation at plant-microbe interaction sites 20 hpi with Bgh.

White line indicates fungal appressorium. ap, appressorium. Bars = 10 µm.