Figure 1.

Arabidopsis GEN1 and SEND1 T-DNA Insertion Mutants.

Structure of GEN1 (A) and SEND1 (B) and the gen1-1 and send1-1 T-DNA insertion mutant alleles. Boxes show exons (unfilled) and 5′ and 3′ untranslated regions (gray fill). The position of the T-DNA insertions is indicated with arrows showing the orientation of the left border and sequences of the T-DNA/chromosome junctions below. The gen1-1 T-DNA insertion is flanked by two left borders (LB1 and LB2) and accompanied by a 3396-bp deletion, which eliminates exons 1 to 4 and a large part of exon 5. A putative in-frame TAA STOP codon in send1-1 is underlined. Numbering under the sequences is relative to the GEN1 or SEND1 start codons. RT-PCR analyses of transcripts of gen1-1 (A) and send1-1 (B). Amplification of the actin transcript (ACT) was used as a control for RT-PCR. The positions and orientations of the PCR primers are shown in the diagrams.