Table 2. Rank (Out of ∼10,000 Contigs Annotated to Arabidopsis Loci) and Expression (RPKM) of the Highest Expressed Acyl Lipid-Related Contigs in Bayberry Knobs.
| Expression Rank | RPKM Sum | Protein Encoded by Arabidopsis Homolog | Pathway/Reaction | ||
|---|---|---|---|---|---|
| Top lipid-related transcripts | |||||
| 1 | 81,312 | Lipid transfer protein (LTP) type 1 (AT2G38540) | Lipid secretion | ||
| 4 | 36,499 | DCR/PEL3/HXXXD acyltransferase | Cutin synthesis | ||
| 8 | 23,603 | sn-2 GPAT4/8 | Cutin synthesis | ||
| 11 | 12,112 | sn-2-GPAT6 | Cutin synthesis | ||
| 19 | 10,147 | GDSL1-motif lipase/transacylase (CuS1/SiCD1) | Cutin synthesis | ||
| 32 | 8,029 | GDSL2-motif lipase/transacylase (AT3G16370) | Cutin synthesis | ||
| 33 | 7,817 | LTP_2 (AT1G48750) | Lipid secretion | ||
| 35 | 7,061 | Keto-acyl CoA synthase (KCS) 10 | Fatty acid elongation | ||
| 36 | 6,926 | KCS19 | Fatty acid elongation | ||
| 37 | 6,705 | LTP_2 (AT3G18280) | Lipid secretion | ||
| 49 | 5,426 | ABCG Transporter 1 (WBC1) | Lipid secretion | ||
| 52 | 4,707 | Acyl carrier protein (ACP) 4 | Fatty acid synthesis | ||
| 54 | 4,705 | Acyl-ACP thioesterase B (FATB) | Fatty acid synthesis | ||
| Transcripts for conventional TAG synthesis | |||||
| 1538 | 418 | Putative diacylglycerol acyltransferase 3 (DGAT3) | |||
| 1613 | 401 | Lysophosphatidic acid acyltransferase 3 (LPAAT3) | |||
| 2372 | 268 | Phosphatidylcholine: diacylglycerol acyltransferase 1 (PDAT1) | |||
| 3960 | 151 | Diacylglycerol acyltransferase 2 (DGAT2) | |||
| 4495 | 126 | sn-1 glycerol-3-phosphate acyltransferase 9 (GPAT9) | |||
| *DGAT1 AND LPAAT 2, 4 were not detected | |||||
The ranks were determined from the RPKM summed across seven developmental stages. Each gene is annotated according to its Arabidopsis homolog. The complete list of genes and developmental profiles are presented in Supplemental Data Set 1.