Figure 9.

Levels of Expression of Genes Encoding Enzymes for the Initial Steps of JA Biosynthesis.

Plants of the indicated genotypes were grown on MS plates for 10 d, then moved to soil and grown for another 10 d before being harvested for total RNA isolation and protein extraction.

(A) Levels of expression of the indicated genes analyzed by quantitative RT-PCR and expressed relative to UBQ10 gene expression. Means ± se for at least three independent plant batches are shown. Significantly higher expression in dgd1-1 or coi1-30 dgd1-1 compared with the wild type or coi1-30, respectively, is indicated as follows: *P < 0.05, **P < 0.01, and ***P < 0.001 (Student’s t test).

(B) Total proteins (10, 20, or 40 μg, indicated above the lanes) were analyzed by SDS-PAGE, followed by immunoblotting with antiserum against cucumber lipid body LOX, Arabidopsis AOC, or pea Toc75.