Figure 2. Intra-oocyte ATP and NADPH contents after mouse COCs maturation in simplified α-MEM containing 5.6-mM glucose and 0.5-mM lactate (GL) in the presence (+) or absence of 150-μM DHEA (D) or 1.5-μM iodoacetate (I) and/or 10-mM F-6-P (F).

For ATP assays, each treatment was repeated 3 times with each replicate containing 60–80 cumulus-free oocytes, and for NADPH assays, each treatment was repeated 3 times with each replicate containing 100–120 cumulus-free oocytes. (a,b) Values without a common letter above their bars differ significantly (P < 0.05).