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. 2016 Feb 9;6:20764. doi: 10.1038/srep20764

Figure 3. Effects of treatment with rotenone, 4-CIN or oxamate during maturation on maturation and blastocyst formation of mouse oocytes.

Figure 3

Panels A and B show percentages of matured (MII) oocytes after COCs were matured with 2-mM pyruvate alone or with different concentrations of rotenone or 4-CIN, respectively. Each treatment was repeated 3 times with each replicate having about 25 oocytes. Panel C shows blastocyst formation after COCs were matured with 2-mM pyruvate (P) or 5.6-mM glucose (G) alone or with (+) 0.025-μM rotenone (R) or 25-μM 4-CIN (C). Each treatment was repeated 4–5 times and each replicate contained around 25 oocytes. Panel D shows percentages of MII oocytes after COCs were matured with 1.5-mM lactate (L) or 2-mM pyruvate (P) alone or in the presence of (+) 30 mM oxamate (O). Each treatment was repeated 3–4 times with each replicate including about 25 oocytes. (a–d) Values without a common letter above their bars differ significantly (P < 0.05).