Table 3. Oocyte maturation and embryo development after COCs were matured in simplified α-MEM containing 2-mM pyruvate (P) alone or with different concentrations of DHEA (D) or iodoacetate (I).
| Supplements | % (No.) MII oocytes | % (No.) Activated oocytes | % (No.) 4-cell embryos | % (No.) Blastocysts |
|---|---|---|---|---|
| P | 97.6 ± 2.4 (121/124)a | 82.3 ± 4.8 (100/121)a | 76.0 ± 4.8 (76/100)a | 29.9 ± 2.6 (30/100)a |
| P + D (150μM) | 100.0 ± 0.0 (120/120)a | 92.7 ± 5.3 (111/120)a | 82.7 ± 3.7 (95/111)a | 32.3 ± 2.5 (36/111)a |
| P + I (1.5μM) | 100.0 ± 0.0 (118/118)a | 88.4 ± 3.7 (106/118)a | 87.1 ± 2.5 (93/106)a | 30.6 ± 2.0 (33/106)a |
| P + I (2 μM) | 100 ± 0.0 (125/125)a | 85.6 ± 3.7 (107/125)a | 77.8 ± 2.4 (83/107)a | 20.5 ± 2.0 (22/107)b |
| P + I (3 μM) | 96.8 ± 2.3 (113/117)a | 93.8 ± 1.8 (106/113)a | 59.5 ± 6.5 (63/106)b | 2.8 ± 1.1 (3/106)c |
a–c: Values without a common letter in their superscripts differ (P < 0.05) in the same column. Pyruvate was used at the optimal concentration (2-mM) selected in a preliminary experiment (data not shown).