Table 4. Oocyte maturation and embryo development after COCs were matured in simplified α-MEM containing 5.6-mM glucose and 0.5-mM lactate in the absence or presence of 150-μM DHEA or 1.5-μM iodoacetate and different concentrations of F-6-P.
| DHEA | F-6-P (mM) | Iodoacetate | % (No.) MII oocytes | % (No.) 4-cell embryos | % (No.) Blastocysts |
|---|---|---|---|---|---|
| — | 0 | — | 98.6 ± 0.9 (141/143)a | 80.7 ± 4.4 (102/126)a | 30.4 ± 2.6 (38/126)a |
| + | 0 | — | 82.9 ± 1.8 (127/153)c | 77.5 ± 3.5 (86/112)a | 12.1 ± 1.5 (13/112)b |
| + | 10 | — | 91.8 ± 3.2 (135/147)a,b | 80.7 ± 3.5 (101/126)a | 29.4 ± 1.8 (37/126)a |
| + | 20 | — | 90.8 ± 2.7 (134/147)b | 64.6 ± 5.5 (76/117)b | 6.0 ± 0.7 (7/117)c |
| + | 10 | + | 81.0 ± 2.7 (128/158)c | 75.0 ± 2.8 (88/116)a,b | 10.3 ± 1.1 (12/116)b,c |
a–c: Values without a common letter in their superscripts differ (P < 0.05) in the same column. Percentages of activated oocytes did not differ among different treatments (data not shown).