Fig. 2.

Survival of D. melanogaster flies carrying or lacking endosymbionts following bacterial infection. Survival of 7-10 day old flies lacking Wolbachia and Spiroplasma (W-S-), flies carrying both endosymbionts (W + S+), and flies carrying Wolbachia only (W + S-) following intrathoracic injection with (a) sterile PBS (septic injury control), (b) E. coli bacteria (strain K12), or (c) P. luminescens bacteria (strain TT01). Survival was monitored for 24 h at 6-h intervals. Data analysis was performed using Log-Rank test (GraphPad Prism5 software) and the values are the percent survival of the infected flies. The means from three independent experiments are shown and bars represent standard errors