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. 2016 Feb 9;16:16. doi: 10.1186/s12866-016-0634-6

Table 1.

Primers used for qRT-PCR analysis

Gene Accession No Primer Sequence Tm (°C)
Cecropin-A1 CG1365 Forward TCTTCGTTTTCGTCGCTCTC 60
Reverse CTTGTTGAGCGATTCCCAGT
Defensin CG1385 Forward CGCATAGAAGCGAGCCACATG 56
Reverse GCAGTAGCCGCCTTTGAACC
Turandot M CG14027 Forward GCTGGGAAAGGTAAATGCTG 61
Reverse AGGCGCTGTTTTTCTGTGAC
Puckered CG7850 Forward GGCCTACAAGCTGGTGAAAG 61
Reverse AGTTCAGATTGGGCGAGATG
RpL32 CG7939 Forward GATGACCATCCGCCCAGCA 61
Reverse CGGACCGACAGCTGCTTGGC

All sequences read 5′ to 3′ left to right