Fig. 1.

The IGF network model qualitatively and quantitatively captured pIGF1R experimental dynamics. a Diagram of molecular events included in the model, double-sided arrows indicate reversible events. b, c Comparison of the best-fit model to experimental results for IGF1- (b) and IGF2- (c) treated OVCAR5. d The calibrated model was used to predict the effect of a mixture of 0.5 nM IGF1 and 0.5 nM IGF2 on pIGF1R in OVCAR5. In these experiments, IGF2 was added directly to the culture media, which contained OVCAR5-secreted IGFBPs. Model results are represented by solid lines and experimental data are represented by circles or squares indicating average ± SD, n = 3