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. 2016 Feb 9;16:58. doi: 10.1186/s12906-016-1036-x

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© Zhang et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effects of Polyphyllin VII (PP7) on mitochondrial membrane potential and intracellular reactive oxygen species (ROS) in HepG2 Cells. a HepG2 cells were treated with indicated concentrations of PP7 for 24 h and photographed using the InCell 2000 confocal microscope after JC-1 staining. b Quantitative analysis of green (FITC)/red (CY3) fluorescence in HepG2 cells, ** p < 0.01, compared to control (CTL). Observation of intracellular ROS generation by DCFH-DA staining in HepG2 cells after treated with indicated doses of PP7 for 24 h (c, e) or treated with 1.32 μM PP7 for different times (d, f). ** p < 0.01, compared to CTL. Data are both represented as means ± SD from 3 independent experiments