Fig. 4.

Effects of Polyphyllin VII (PP7) on the MAPK and PTEN/p53 pathways in HepG2 cells. Key proteins in MAPK pathways, PTEN and p53 were determined by Western blotting analysis after treating the cells with PP7 at indicated concentrations for 24 h. The indicated number under each band was the fold changes of expression level compared to that in the control group. GAPDH was used as the internal standard for protein loading