Fig. 5.

Inhibition of MAPK pathways and ROS production attenuated PP7-induced apoptosis and necrosis in HepG2 cells. a Effects of JNK inhibitor SP600125, ERK inhibitor PD98059 and p38 inhibitor SB203580 on the expression of apoptosis-related proteins in PP7-treated cells by Western blotting analysis. The indicated number under each band was the fold changes of expression level compared to that in the control group. GAPDH was used as the internal standard for protein loading. b Effects of SP600125 (SP) , PD98059 (PD), SB203580 (SB) and NAC on PP7-induced apoptosis and necrosis tested by Hoechst 33342, Annexin V and PI staining. c Quantification of apoptotic cells in (b). * p < 0.05, ** p < 0.01, versus control (CTL). Data are represented as means ± SD from 3 independent experiments