Table 2.

The top 20 hypomethylated a-CpGs in middle-aged individuals. The hypermethylated and hypomethylated a-CpGs are shown separately in Tables 1 and 2, respectively. The top-ranking hypomethylated a-CpGs were selected with the following criteria: 1) direction of the association based on the value of beta regression (denoted as ‘betareg’) estimate of age; 2) more than one hit identified per gene (q-value < 0.05 which corresponds to false discovery rate < 5 %) and 3) the top-ranking p-values. The full list of a-CpGs is shown in Additional file 3: Table S4. The q-value denotes the Benjamini-Hochberg-corrected p-value

ProbeID	Gene name	CHR	Coordinate	Betareg estimate of age	q-value
cg00791074	MTHFD1L	6	151227862	-0.018	7.51E-04
cg18618815	COL1A1	17	45630323	-0.018	5.99E-03
cg14169886	PRDM16	1	3101709	-0.014	5.99E-03
cg01820374	LAG3	12	6752344	-0.014	9.24E-03
cg19421125	LAG3	12	6753117	-0.022	1.02E-02
cg14829066	NTRK3	15	86360145	-0.013	1.49E-02
cg03290281	C6orf195	6	2577606	-0.021	1.49E-02
cg05561193	DCLK2	4	151218492	-0.017	1.96E-02
cg20249566	NWD1	19	16691739	-0.024	1.97E-02
cg23928726	PEX10	1	2334858	-0.014	1.97E-02
cg20007894	SCAND3	6	28648421	-0.019	2.08E-02
cg16355231	PEX10	1	2334839	-0.019	2.14E-02
cg15058210	HDAC4	2	239861814	-0.018	2.16E-02
cg06030846	TMEM108	3	134581182	-0.011	2.16E-02
cg25994988	UBASH3B	11	122157592	-0.011	2.16E-02
cg18345924	NCAM2	21	21294102	-0.016	2.18E-02
cg00638021	COL1A1	17	45622061	-0.013	2.26E-02
cg19344626	NWD1	19	16691749	-0.024	2.36E-02
cg01288258	ITFG2	12	2792128	-0.011	2.41E-02
cg05221385	TAF10	11	6590080	-0.010	2.43E-02