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. 2016 Feb 8;13:32. doi: 10.1186/s12974-016-0488-8

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© Lee et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Immunofluorescence staining of Iba-1 (a, d red) in microglia and GFAP (b, e green) and merged images (c, f) in astrocytes in the microglial (a–c) and astrocytic cultures (d–f). The results suggested that more than 99 % of cells prepared from human brains are microglia and astrocytes, respectively. The calibration bar in F: 50 μm