Figure 6.
Effect of B.suis.S2 on the functions of GTCs. (A) The migration ability of B.suis.S2 (MOI = 100:1)-infected GTCs was measured using a Transwell chamber migration assay. Crystal violet was used to stain the migrated cells (bar = 10 μm). The data shown are representative of 4 independent experiments. (B) The invasion ability of B.suis.S2 (MOI = 100:1)-infected GTCs was measured using a Matrigel invasion chamber assay. Crystal violet was used to stain the invaded cells (bar = 20 μm). The data shown are representative of 4 independent experiments. (C) The mRNA expression levels of MMP2 and MMP9 are shown for the control at 24 h. The results of real-time PCR (the data are corrected for expression of the housekeeping gene GAPDH; mean ±standard deviations from 5–6 independent experiments, P < 0.05).