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Expression of L-R patterning genes was unaffected in Foxh1mEH1/mEH1 embryos. (A–F) Whole-mount in situ hybridization analysis of L-R patterning genes (A,B) Nodal, (C,D) Lefty2, and (E,F) Pitx2 in the left LPM of control or Foxh1mEH1/mEH1 embryos at E8.25. No aberrant expression of L-R patterning genes was detected in mutant embryos. Black and red arrowheads indicate midline and LPM, respectively. Embryos orientations: (A–F) anterior views. Scale bars: 200 μm. L, left; R, right.
