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. Author manuscript; available in PMC: 2016 Feb 9.
Published in final edited form as: Biomaterials. 2014 Apr 8;35(21):5491–5504. doi: 10.1016/j.biomaterials.2014.03.039

Fig. 1.

Fig. 1

A) Reaction scheme showing surface functionalization of PLGA microparticle covalently with a cationic polymer, PEI through EDC/NHS chemistry; B) Diagram showing surface loading of various anionic nucleic acid based immunomodulatory molecules e.g., CpG, Poly (I:C), siRNA, pDNA on cationic PLGA-PEI microparticles by electrostatic interaction; C) Schematic illustrating binding of soluble CpG to TLR9 receptor of DCs leading to secretion of both IL12 and IL10 cytokines, which may ultimately polarize to Th1 due to IL12, and Th2/Treg due to IL10; D) Schematic showing the concept of co-delivery IL10 siRNA and CpG molecules surface loaded on cationic PLGA microparticle to DCs to switch CpG induced IL12/IL10 (Th1/Th2) cytokine balance by inhibiting IL10 secretion by silencing IL10 gene in DCs and ultimately resulting into an enhanced Th1 response.