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. Author manuscript; available in PMC: 2016 Feb 9.
Published in final edited form as: Mol Microbiol. 2009 Feb 11;72(1):85–97. doi: 10.1111/j.1365-2958.2009.06625.x

Fig. 2. Inhibition of bacterial growth and lipid synthesis by Ro 48-8071.

Fig. 2

Panel A: Inhibition of M. tuberculosis growth was determined in 96 well plates using 7H9 medium (supplemented with oleic acid, albumin, dextrose and 0.05% Tween 80). Ro 48-8071 was added at the indicated concentrations; the structure of Ro 48-8071 is inset. Panel B: TLC analysis of neutral lipids isolated from M. bovis BCG after labeling with L-[methyl-14C]methionine in the presence of the indicated concentrations of Ro 48-8071. Cells were pre-incubated with Ro 48-8071 for 20 min at 37°C followed by labeling for 2 h. at the same temperature. The arrow indicates the material that was isolated for analysis by mass spectrometry.