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. Author manuscript; available in PMC: 2016 Feb 9.
Published in final edited form as: Mol Microbiol. 2009 Feb 11;72(1):85–97. doi: 10.1111/j.1365-2958.2009.06625.x

Fig. 4. Inhibition of MenA activity by Ro 48-8071.

Fig. 4

Reaction mixtures contained 500 μM DHNA, 10 μM [3H]farnesyl diphosphate, 5 mM MgCl2 and 0.1% CHAPs in 100 mM Tris-HCl (pH 8.0) and 50–100 μg membrane protein. Ro 48-8071 was added at the indicated concentrations. Reactions were stopped and the product analyzed as described in the Experimental Procedures. Data presented are average values of triplicate determinations.