FAMEs (and MAMEs) of [1,2-14C]acetate-labeled M. tuberculosis and M. bovis BCG were prepared from untreated and ISO-treated cultures, and equal counts were subjected to radio-GC coupled to the Lablogic GC-RAM radio detector under conditions that resolve only the FAMEs. A, untreated M. tuberculosis culture; B, ISO-treated (2.0 μg/ml) M. tuberculosis culture; C, untreated M. bovis BCG culture; D, ISO-treated (2.0 μg/ml) M. bovis BCG culture. TBSA, tuberculostearic acid.