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Flushing of bone marrow by centrifugation. Remove lid from 0.5 ml eppendorf tube and puncture the base of the eppendorf tube with a 27-gauge needle. Place this microtube in a 1.5 ml centrifuge tube on ice with 500 μl of PBS. Dissect long bones, removing fat and muscles, and place both tibia and femur in the 0.5 ml eppendorf tube, with open ends at the bottom of the tube. Isolate bone marrow by centrifuging at full speed (13 000 r.p.m.) for 2 min. Wash bone marrow lysates with PBS.
