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. 2015 Oct 20;6(39):41464–41478. doi: 10.18632/oncotarget.6203

Figure 2. Analysis of the effect of alcohol and oleate on lipid peroxidation and pro-inflammatory gene expression.

Figure 2

PHH were pre-incubated with 0.2mM oleate (Ol) or BSA (served as control [Ctrl]) for 24h. Subsequently, cells were co-incubated with 50mM alcohol (Alc) for additional 24h. A. Analysis of cellular mRNA levels of CPT-1 and Acox-1 by quantitative RT- PCR. B. Analysis of cellular MDA levels by TBARS assay. C. Analysis of HMOX-1 and p47phox mRNA levels by quantitative RT-PCR. D. Analysis of p-IκBα and p-p65 phosphorylation by Western blot analysis. Actin served as control for loading adjustment. E. Analysis of IL-8 and ICAM-1 mRNA levels by quantitative RT-PCR. (*: p < 0.05 compared to control; #: p < 0.05 compared to oleate or alcohol condition).