Skip to main content
. 2015 Oct 20;6(39):41464–41478. doi: 10.18632/oncotarget.6203

Figure 6. Analysis the effect of autophagy inhibition or induction on the effects of alcohol and oleate on lipid accumulation, lipid peroxidation and inflammation.

Figure 6

HepG2 E47 cells were pre-incubated with 0.2mM oleate (Ol) or BSA (served as control) for 24h. Subsequently, cells were co-incubated with 3-methyl adenine (3-MA), an autophagy inhibitor (2.5mM) or rapamycin (0.2μg/ml) for 1 h before adding 50mM alcohol (Alc) to cultured medium for additional 24 h. A., D. Cellular triglyceride content normalized to total cellular protein. B., E. Analysis of cellular MDA levels by TBARS assay. C., F. Analysis of ICAM-1 mRNA levels by quantitative RT-PCR analysis. (*: p < 0.05 compared to corresponding control; #: p < 0.05 compared to corresponding oleate or alcohol condition; $: p < 0.05 compared to the equal condition in control group).