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. 2015 Nov 2;6(39):41479–41496. doi: 10.18632/oncotarget.6286

Figure 1. Effect of PA and MA in TG levels and ceramide species.

Figure 1

PMH were incubated with PA, MA or OA and the combination PA plus MA (PM) and PA plus OA (PO) at 0.5mM overnigh. PMH were processed for oil-red staining A. and for the determination of TG levels at the concentrations indicated B. In some cases, PMH were treated with 0.5mM PA and with increasing MA doses as indicated to determine total ceramide levels C. Moreover, lipid extracts were analyzed by mass spectrometry to determine the relative abundance of different ceramide species D. Results are the mean±SEM of N = 5-6 individual experiments. *p < 0.05 vs. control and PA-treated PMH.