Figure 3. Effect of the antioxidant BHA and the SPT inhibitor myriocin in PA and PA plus MA-induced reactive oxygen species and cell death.

PMH were treated with PA with or without MA (0.5mM each) for 12 hours. Cell death was then evaluated by trypan blue exclusion A. In some cases, cells were incubated with BHA (100μΜ) in the presence of PA and PA plus MA and ROS generation by DCF B. and cell death C. were determined. Alternatively, PMH were treated with myriocin (20μΜ) to block de novo ceramide synthesis and its effect on ROS generation D. and cell death E. were analyzed. Results are the mean±SEM of N = 5-6 individual experiments. *p < 0.05 vs. control and PA-treated PMH.