Figure 5. Effect of myriocin on total hepatic ceramide levels and ER stress.

PMH were incubated with PA with or without MA (0.5mM) each for 6 hours in the presence of myriocin (20μM) and processed for total ceramide determination by HPLC A. Alternatively, expression of CHOP B. and sXBP-1 C. was examined by RT-PCR with or without myriocin. Results are the mean±SEM of N = 5-7 individual experiments. *p < 0.05 vs. control and PA-treated PMH.